DNA Extraction Service Both laborious and time-consuming, DNA extraction is a basic technology in the biology lab and is essential for all downstream genomic analyses. LifeScience can separate high-quality DNA from a wide variety of specimens such as viruses, fungi, bacteria, plants, blood, saliva, and insects. High-quality DNA can be used directly in enzyme digestion, … Continue reading DNA Extraction Service
Full-length cDNA Library Construction A cDNA library is the collection of cDNA clones representing a stage of given cells or tissues, which contains not only gene information but their abundance information. Full-length cDNA molecules are fully reverse-transcripted from intact mRNA molecules and contain the full length of the protein coding region. cDNA library can be … Continue reading Full-length cDNA Library Construction
Genome-Walking Cloning Service Genome walking determines the genome DNA sequence that flanks two ends of a known sequence. Genome DNA walking is capable of obtaining several kb of sequence in a single round. This is ideal for identifying gene promoter regions, filling the gap in genome sequences, and obtaining the whole length of target … Continue reading Genome-Walking Cloning Service
Genotyping Services Genotyping determines differences in genome by examining their sequence. It reveals the alleles which have been inherited from their parents. Genotyping can be grouped into four categories: biochemistry-based assay, enzymes digestion-based genotyping, PCR-based genotyping, and DNA sequencing-based genotyping. LifeScience provides SNPs, microsatellites(SSR, STR), and sequencing-based genotyping. SNP genotyping is the third generation of … Continue reading Genotyping Service
Microbe Identification Eukaryotes Identification 23S-5S-16S ITs PCR amplification and sequencing is an effective method for identifying bacterial species. 23S-5S-16S ITs amplification and NGS analysis can be used to profile microbe populations and their abundances in environmental samples. Procedures: Provide pure cultures or environmental samples from various sources Basic services include (for pure cultures, Sanger sequencing) … Continue reading Microbe Identification
mRNA amplification LifeScience provides full-length mRNA amplification services that cater to high quantity mRNA requirements of experiments such as cDNA libraries construction, transcriptome sequencing, and Northern blotting, from limited quantity samples. The T7 promoter is incorporated into the newly synthesized double-strand cDNA molecules which are used as templates to transcript mRNA molecules by T7 … Continue reading mRNA Amplification
NGS & Bioinfor. Analysis LifeScience provides various NGS services from sample preparation to data analysis on NovaSeq 6000 platform. NovaSeq 6000 platform which has the ability to generate up to 6 Tb and 20 billion reads in dual flow cell mode is more efficient and cost-effective than ever. Our services include Genome-Wide Sequencing, Transcriptome sequencing, … Continue reading NGS & Bioinfor. Analysis
Normalized full-length cDNA library construction A normalized full-length cDNA library normalizes the abundance of each gene. This means that all genes have similar copy numbers in the library. Before cDNA is ligated into the vector, the incorporated normalizing procedure can reduce the gene redundancy in the final libraries, which ensures that genes with low copy … Continue reading Normalized full-length cDNA library construction
Phage display library construction Phage display library technology, which uses bacteriophage to connect proteins with genetic information, is used to study protein/protein and protein/peptide interactions. Gene encoding a protein/peptide of interest is inserted into a phage capsid gene, causing the phage to display the protein/peptide on its capsid, which results in a connection between genotype with phenotype. The displaying phages can be used … Continue reading Phage Display Library Construction
Plasmid extraction Services LifeScience Plasmid DNA Preparation Service accommodates both small research labs as well as large-scale manufacturing biotech and pharmaceutical companies. Specific yields for our plasmid DNA preparation service range from micrograms to milligrams. Scale Culture volume Yields Mini Prep 10 mL - 50 mL 15 µg - 50 µg Media Prep 100 mL - … Continue reading Plasmid Extraction Services
RACE Services Rapid Amplification of cDNA Ends (RACE) amplifies unknown sequences of nucleic acid from a messenger RNA template between a defined internal site at both 3' and 5' ends of the mRNA molecule. It is an effective method of obtaining a whole-length cDNA sequence from a partially known sequence. Next-generation sequencing generates large amounts … Continue reading RACE Cloning Service
Plant RNA Isolation Services Isolating high-quality RNA from plant and fungi specimens contaminated heavily by polysaccharides, proteins, and polyphenols is a tough task for researchers, especially those who have no experience in removal of contaminants. However, the quality of RNA is the key element in downstream experiments such as RACE cloning, cDNA library construction, … Continue reading RNA Extraction Service
Sanger Sequencing With over 15 years’ experience in Sanger sequencing, LifeScience conducts Sanger sequencing services using BigDye v3.1 reagents on an ABI 3730XL. LifeScience provides express sequencing, standard sequencing, and lab intensive sequencing. With express sequencing, customers prepare their samples (plasmid, cleaned PCR product, BAC) and adjust the concentration between the required ranges in … Continue reading Sanger Sequencing
T-vector cloning T-vector cloning is a basic cloning technology widely used to produce recombinant plasmids for sequencing. Compared with PCR product sequencing, T-vector cloning sequencing has a high sequencing success rate and precise sequencing results. LifeScience provides T-vector cloning services to our customers. PCR products are ligated onto a T-vector after performing gel recovery … Continue reading T-Vector Cloning
Yeast one/two-hybrid library construction LifeScience, a leading biotechnology company, provides high-quality yeast two/one-hybrid cDNA library construction services for research communities worldwide. Our success in this area comes from our experience in total RNA extraction, reverse transcription, and recombinant vector preparation. With years of experience in the library and yeast one/two-hybrid library construction field, LifeScience can … Continue reading Yeast One/Two-hybrid Library Construction